How does CRISPR-Cas9 achieve targeted genome editing?

How does CRISPR-Cas9 achieve targeted genome editing?

• A. Cas9 nuclease guided by a short RNA creates a double-strand break at a specific genomic sequence; repair either uses a template or non-homologous end joining to introduce edits.
• B. Cas9 integrates into the genome and creates single-strand breaks at random locations.
• C. CRISPR-Cas9 acts as a DNA ligase sealing breaks without sequence specificity.
• D. Cas9 acts as a reverse transcriptase to copy RNA into DNA.

Answer: (A)